Seminar Details

Infrared Photodissociation Spectroscopy of Biomolecular Ions

Kerim Gulyuz, University of Florida
Friday, May 31, 10:00 AM - Special Seminar
NSCL Seminar Room

Mass spectrometry (MS) is a very important analytical tool in identification of molecules. However, it gives limited structural information and therefore needs to be complemented by another technique. On the other hand, infrared multiple photon dissociation (IRMPD) spectroscopy has been demonstrated to be useful at distinguishing putative chemical structures by virtue of diagnostic vibrations. This approach has been particularly successful in the characterization of dissociation products from collision-induced dissociation (CID) of protonated peptides. Most such studies have been conducted at free electron laser (FEL) facilities, which are ideally suited to IRMPD spectroscopy in the mid-infrared region (500-2000 cm-1) [1]. We have recently extended this approach to the hydrogen stretching region (3000-4000 cm-1) [2], which is particularly relevant in verifying proton/charge location. A custom-built hybrid mass spectrometer [3], that combines MS and IRMPD, will be presented, where mass-selected, covalently-bound ions are photodissociated in a reduced-pressure quadrupole ion trap using a tunable optical parametric oscillator laser.